Journal: Journal of Biological Chemistry

Article Title: High Resolution Mapping of the Binding Site on Human IgG1 for FcγRI, FcγRII, FcγRIII, and FcRn and Design of IgG1 Variants with Improved Binding to the FcγR

doi: 10.1074/jbc.m009483200

Figure Lengend Snippet: FIG. 1. Binding of anti-IgE E27 IgG1 to human FcgR. A, binding of E27 monomers (solid circles), dimeric (solid squares), and hexameric (open squares) complexes to FcgRIIA-Arg131. B, binding of E27 mono- mers (solid circles), dimeric (solid squares), and hexameric (open squares) complexes to FcgRIIIA-Phe158. Dimers were formed by mixing E27 IgG1 and a F(ab9)2 fragment of goat anti-human k light chain at 1:0.5 molar ratio at 25 °C for 1 h (50). Hexameric complexes (i.e. trim- eric in E27 IgG1) were formed by mixing E27 IgG1 with human IgE in a 1:1 molar ratio at 25 °C for 1 h (36).

Article Snippet: Plates were blocked with assay buffer at 25 °C for 1 h. Serial 3-fold dilutions of monomeric E27 and variants (10.0–0.0045 mg/ml) were added to plates and incubated for 2 h. After washing plates with assay buffer, IgG bound to FcgRI was detected with peroxidase-conjugated F(ab9)2 fragment of goat anti-human F(ab9)2-specific IgG (Jackson ImmunoResearch) or with peroxidase-conjugated protein G (Bio-Rad).

Techniques: Binding Assay

Journal: Cell metabolism

Article Title: CD38-NAD + Axis Regulates Immunotherapeutic Anti-Tumor T Cell Response

doi: 10.1016/j.cmet.2017.10.006

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-Rabbit Alexa647 , Jackson ImmunoResearch Laboratories , Clone: N/A; Cat# 111-607-003; RRID: AB_2338084.

Techniques: Recombinant, Staining, Cell Isolation, Synthesized, Lysis, Extraction, Cell Based Assay, Activity Assay, Enzyme-linked Immunosorbent Assay, Immunoprecipitation, cDNA Synthesis, SYBR Green Assay, Microarray, Knock-In, Plasmid Preparation, Software